Coding

Part:BBa_K4769221:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


Porphyran utilisation locus fragment 4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 815
    Illegal EcoRI site found at 869
    Illegal EcoRI site found at 1646
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 815
    Illegal EcoRI site found at 869
    Illegal EcoRI site found at 1646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 815
    Illegal EcoRI site found at 869
    Illegal EcoRI site found at 1646
    Illegal XhoI site found at 836
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 815
    Illegal EcoRI site found at 869
    Illegal EcoRI site found at 1646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 815
    Illegal EcoRI site found at 869
    Illegal EcoRI site found at 1646
    Illegal AgeI site found at 6160
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1401
    Illegal SapI.rc site found at 5998


Design Notes

N/A


Source

P. plebeius genome

Primers for cloning: FW: cttttaataatataaatatatgaacttcagatataaaacaatagtattcag (+ desired overhangs) RV: ttattttctcactttcttatcgtagcgt (+ desired overhangs)

References